DNA

Part:BBa_K3148002:Design

Designed by: YiYang Jiang   Group: iGEM19_Shenzhen_SFLS   (2019-10-16)


Mgfp5-Masp1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 358
    Illegal PstI site found at 308
    Illegal PstI site found at 398
    Illegal PstI site found at 500
    Illegal PstI site found at 602
    Illegal PstI site found at 794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 358
    Illegal PstI site found at 308
    Illegal PstI site found at 398
    Illegal PstI site found at 500
    Illegal PstI site found at 602
    Illegal PstI site found at 794
    Illegal NotI site found at 799
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 76
    Illegal BamHI site found at 544
    Illegal BamHI site found at 841
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 358
    Illegal PstI site found at 308
    Illegal PstI site found at 398
    Illegal PstI site found at 500
    Illegal PstI site found at 602
    Illegal PstI site found at 794
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 358
    Illegal PstI site found at 308
    Illegal PstI site found at 398
    Illegal PstI site found at 500
    Illegal PstI site found at 602
    Illegal PstI site found at 794
    Illegal NgoMIV site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the most important design consideration is the protein linker used to connect the proteins. Thanks to professor Zhong's research, we derive the correct linker to use.


Source

The mgfp5 protein is source from the genomics of mussel. Although Mgfp5 is a recombinant protein of mfp5. But the original source is indeed the genomics of mussel The masp1 protein is sourced from the genomics of spiders.

References